Hotspot

accD-psaI

LSC · 38476-39205 · 730 bp

Back to Chamaegastrodia

Pi 0.107

MarkerSeek score 83.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 74
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACAGTACCCGACGGTTTAC	AATCGGTCATAACCACTGCC	55.0	50.0	58.0	58.0	1585-1672 bp	1.0	89.0
accD-psaI_p2	CTACATTGCGTTTACGGGGA	AATCGGTCATAACCACTGCC	50.0	50.0	58.0	58.0	1633-1720 bp	1.0	88.8
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	AATCGGTCATAACCACTGCC	55.0	50.0	58.0	58.0	1701-1788 bp	1.0	88.4
accD-psaI_p4	CCTACAACTGGCGGAGTTAC	AATAGACCCGCGATCCCATA	55.0	50.0	58.0	58.1	1641-1728 bp	1.0	88.4
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	CGGTCATAACCACTGCCTAC	55.0	55.0	58.0	58.1	1698-1785 bp	1.0	88.3