Pi
0.031
MarkerSeek score
70.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | TCGGAATCGAACCAATGACC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 837-1076 bp | 1.0 | 82.7 |
| trnT-UGU-trnL-UAA_p2 | TCGGAATCGAACCAATGACC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.0 | 58.1 | 845-1084 bp | 1.0 | 82.2 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 803-1042 bp | 1.0 | 81.9 |
| trnT-UGU-trnL-UAA_p4 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 811-1050 bp | 1.0 | 81.4 |
| trnT-UGU-trnL-UAA_p5 | TCGGAATCGAACCAATGACC | ATCCAATCAAGTCCGTAGCG | 50.0 | 50.0 | 58.0 | 57.8 | 860-1099 bp | 1.0 | 80.9 |