Hotspot

trnY-GUA-trnE-UUC

LSC · 18570-18628 · 59 bp

Back to Chamaegastrodia

Pi 0.0847

MarkerSeek score 71.80

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 5
pi: 0.0847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TCTTGCTTGGGCTGCTTTAA	CCATGGCGTTACTCTACCAC	45.0	55.0	58.0	58.1	1004-1248 bp	1.0	82.8
trnY-GUA-trnE-UUC_p2	TCTTGCTTGGGCTGCTTTAA	TGGCGTTACTCTACCACTGA	45.0	50.0	58.0	58.1	1001-1245 bp	1.0	82.7
trnY-GUA-trnE-UUC_p3	GGACTTGAGTAAGAGTGGCG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	559 bp	0.5	59.5
trnY-GUA-trnE-UUC_p4	GGACTTGAGTAAGAGTGGCG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	556 bp	0.5	59.3
trnY-GUA-trnE-UUC_p5	GGACTTGAGTAAGAGTGGCG	CCATTGGAACGGAACCAGAA	55.0	50.0	58.0	58.1	992 bp	0.5	59.2