Hotspot

trnD-GUC-trnY-GUA

LSC · 18191-18485 · 295 bp

Back to Chamaegastrodia

Pi 0.0461

MarkerSeek score 70.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 13
pi: 0.0461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGTGCTCTAACCGATTGAACT	CTGGTTCAAATCCAGCTCGG	47.6	55.0	58.0	58.9	335-348 bp	1.0	70.6
trnD-GUC-trnY-GUA_p2	GGTGCTCTAACCGATTGAACT	GAGATGTCTACGCTGGTTCA	47.6	50.0	58.0	57.1	347-360 bp	1.0	70.1
trnD-GUC-trnY-GUA_p3	GGTGCTCTAACCGATTGAACT	TCTACGCTGGTTCAAATCCA	47.6	45.0	58.0	56.9	341-354 bp	1.0	68.6
trnD-GUC-trnY-GUA_p4	GGTGCTCTAACCGATTGAACT	TGGTTCAAATCCAGCTCGG	47.6	52.6	58.0	57.8	334-347 bp	1.0	67.9
trnD-GUC-trnY-GUA_p5	GGTGCTCTAACCGATTGAACT	GAGATGTCTACGCTGGTTCAA	47.6	47.6	58.0	57.8	347-360 bp	1.0	67.8