Hotspot

rpoC2-trnC-GCA

LSC · 14939-16938 · 2000 bp

Back to Chamaegastrodia

Pi 0.406
MarkerSeek score 79.10
Primer available yes
Label rpoC2-trnC-GCA

Feature Metadata

label_name
rpoC2-trnC-GCA
feature_type
igs
region
LSC
length_bp
2000
variable_sites
803
pi
0.406
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-trnC-GCA_p1 GTTTTGGCAGGCCTACTCAT TTGGTACCTGTTTGAGCCAC 50.0 50.0 58.2 58.0 2248 bp 0.5 58.5
rpoC2-trnC-GCA_p2 GTTTTGGCAGGCCTACTCAT CAGAGCCTCTCGTGAGTCTA 50.0 55.0 58.2 58.0 2642 bp 0.5 58.5
rpoC2-trnC-GCA_p3 GTTTTGGCAGGCCTACTCAT TACTCAGAGCCTCTCGTGAG 50.0 55.0 58.2 58.0 2646 bp 0.5 58.5
rpoC2-trnC-GCA_p4 GTTTTGGCAGGCCTACTCAT TGAGTACTCAGAGCCTCTCG 50.0 55.0 58.2 58.0 2650 bp 0.5 58.5
rpoC2-trnC-GCA_p5 GTTTTGGCAGGCCTACTCAT GAGTACTCAGAGCCTCTCGT 50.0 55.0 58.2 58.0 2649 bp 0.5 58.5