Hotspot

rps16-trnQ-UUG

LSC · 4585-4815 · 231 bp

Back to Chamaegastrodia

Pi 0.0696

MarkerSeek score 69.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 16
pi: 0.0696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	304-323 bp	1.0	82.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GGGCAATTTGGCGAAGAAAA	45.0	45.0	58.1	57.8	778-821 bp	1.0	82.9
rps16-trnQ-UUG_p3	GTGTTTCCTTGTTCCGGGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	688 bp	0.5	58.6
rps16-trnQ-UUG_p4	GTGTTTCCTTGTTCCGGGAT	GGGCAATTTGGCGAAGAAAA	50.0	45.0	58.1	57.8	1162 bp	0.5	57.9
rps16-trnQ-UUG_p5	GTGTTTCCTTGTTCCGGGAT	TCGCTTTTTGAATCGAGGGT	50.0	45.0	58.1	57.8	1395 bp	0.5	57.8