Hotspot

rpl16

LSC · 82603-84110 · 1508 bp

Back to Chamaedorea

Pi 0.0132

MarkerSeek score 60.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1508
variable_sites: 38
pi: 0.0132
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCCTTTTGTTTAGTTCTACATCC	GGTTGAATAAAAATTCGATTGACCA	37.5	32.0	56.6	56.9	1533-1572 bp	1.0	44.8
rpl16_p2	TCATCCTCAATATCCCTTTTGT	AGGTTGAATAAAAATTCGATTGACCA	36.4	30.8	54.8	58.1	1546-1585 bp	1.0	44.7
rpl16_p3	TCATCCTCAATATCCCTTTTGT	GGTTGAATAAAAATTCGATTGACCA	36.4	32.0	54.8	56.9	1545-1584 bp	1.0	44.7
rpl16_p4	TCCCTTTTGTTTAGTTCTACATCC	AGGTTGAATAAAAATTCGATTGACCA	37.5	30.8	56.6	58.1	1534-1573 bp	1.0	44.7
rpl16_p5	TCATCCTCAATATCCCTTTTGTT	AGGTTGAATAAAAATTCGATTGACCA	34.8	30.8	55.6	58.1	1546-1585 bp	1.0	44.7