Hotspot

ndhF-rpl32

SSC · 114776-115070 · 295 bp

Back to Chamaedorea

Pi 0.0297

MarkerSeek score 56.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 295
variable_sites: 15
pi: 0.0297
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCATATTGATATGTATGTTCCA	ACCGATTTTGCTAAAGAAAAAGC	30.4	34.8	53.7	56.8	370-459 bp	1.0	46.8
ndhF-rpl32_p2	TGCATATTGATATGTATGTTCCA	ACCGATTTTGCTAAAGAAAAAGCT	30.4	33.3	53.7	58.2	370-459 bp	1.0	46.8
ndhF-rpl32_p3	TGCATATTGATATGTATGTTCCA	CCGATTTTGCTAAAGAAAAAGCT	30.4	34.8	53.7	56.6	369-458 bp	1.0	46.8
ndhF-rpl32_p4	TGCATATTGATATGTATGTTCCA	AACCGATTTTGCTAAAGAAAAAGC	30.4	33.3	53.7	57.5	371-460 bp	1.0	46.8
ndhF-rpl32_p5	TGCATATTGATATGTATGTTCCA	GGTAGAAACCGATTTTGCTAAAGA	30.4	37.5	53.7	57.5	377-466 bp	1.0	46.7