Hotspot

rps3-rpl22

LSC · 84938-85003 · 66 bp

Back to Chamaedorea

Pi 0.0384

MarkerSeek score 56.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 4
pi: 0.0384
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CCAAGTCTGAAACCAAGTGGA	AAAGTTAAGACCTCGGGCTC	47.6	50.0	58.1	57.2	194-198 bp	1.0	68.6
rps3-rpl22_p2	CCAAGTCTGAAACCAAGTGGA	AAAAGTTAAGACCTCGGGCTC	47.6	47.6	58.1	57.9	195-199 bp	1.0	66.2
rps3-rpl22_p3	CCAAGTCTGAAACCAAGTGG	AAAGTTAAGACCTCGGGCTC	50.0	50.0	56.5	57.2	194-198 bp	1.0	65.5
rps3-rpl22_p4	CCAAGTCTGAAACCAAGTGGA	AAAAGTTAAGACCTCGGGCT	47.6	45.0	58.1	56.8	195-199 bp	1.0	64.7
rps3-rpl22_p5	CCAAGTCTGAAACCAAGTGG	AAAAGTTAAGACCTCGGGCTC	50.0	47.6	56.5	57.9	195-199 bp	1.0	63.2