Hotspot

psaJ-rpl33

LSC · 68297-68843 · 547 bp

Back to Chamaedorea

Pi 0.0124

MarkerSeek score 58.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 15
pi: 0.0124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCCTTGTCATTCCCCTT	TGACACCATTTCGGACACAA	50.0	45.0	57.9	57.7	636-647 bp	1.0	79.4
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	TTGACACCATTTCGGACACA	50.0	45.0	57.9	57.7	637-648 bp	1.0	79.4
psaJ-rpl33_p3	GATGCCTTGTCATTCCCCTT	ACACCATTTCGGACACAACT	50.0	45.0	57.9	57.7	634-645 bp	1.0	79.3
psaJ-rpl33_p4	GATGCCTTGTCATTCCCCTT	ACCATTTCGGACACAACTGG	50.0	50.0	57.9	58.4	632-643 bp	1.0	78.9
psaJ-rpl33_p5	GATGCCTTGTCATTCCCCTT	CCATTTCGGACACAACTGGT	50.0	50.0	57.9	58.4	631-642 bp	1.0	78.9