Hotspot

ndhC-trnV-UAC

LSC · 51362-52332 · 971 bp

Back to Chamaedorea

Pi 0.0183

MarkerSeek score 66.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 37
pi: 0.0183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	1101-1273 bp	1.0	84.4
ndhC-trnV-UAC_p2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1093-1265 bp	1.0	84.3
ndhC-trnV-UAC_p3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	1183-1355 bp	1.0	82.0
ndhC-trnV-UAC_p4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1175-1347 bp	1.0	82.0
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	1100-1272 bp	1.0	81.7