Hotspot

trnT-GGU-psbD

LSC · 32633-33661 · 1029 bp

Back to Chamaedorea

Pi 0.00982

MarkerSeek score 57.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 22
pi: 0.00982
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1180-1183 bp	1.0	78.9
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1175-1178 bp	1.0	78.8
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.3	57.7	1138-1141 bp	1.0	77.4
trnT-GGU-psbD_p4	AAACGGGCCCTTTTAACTCA	CCCTCCGTAACCAATCATCC	45.0	55.0	57.6	57.7	1181-1184 bp	1.0	76.7
trnT-GGU-psbD_p5	GCCATGGTAAGGCGTAAGTC	CCCTCCGTAACCAATCATCC	55.0	55.0	58.7	57.7	1148-1151 bp	1.0	74.1