Hotspot

atpH-atpI

LSC · 14243-15074 · 832 bp

Back to Chamaedorea

Pi 0.0161

MarkerSeek score 61.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 31
pi: 0.0161
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	884-941 bp	1.0	80.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	912-969 bp	1.0	80.4
atpH-atpI_p3	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	967-1024 bp	1.0	80.0
atpH-atpI_p4	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	58.0	58.6	995-1052 bp	1.0	79.9
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	968-1025 bp	1.0	79.5