Hotspot

accD-ycf1

Genome · 114697-116162 · 1466 bp

Back to Chamaecyparis

Pi 0.16

MarkerSeek score 81.60

Primer available yes

Label accD-ycf1

Feature Metadata

label_name: accD-ycf1
feature_type: igs
region: Genome
length_bp: 1466
variable_sites: 406
pi: 0.16
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-ycf1_p1	AGTATGGGATCGGTAGTGGG	ACGTGAGGTTTCCAAATGCT	55.0	45.0	58.0	58.0	2197-2609 bp	1.0	91.8
accD-ycf1_p2	GTTCATGGGAGGCAGTATGG	ACGTGAGGTTTCCAAATGCT	55.0	45.0	58.0	58.0	2210-2622 bp	1.0	91.6
accD-ycf1_p3	TCAGTTCATGGGAGGCAGTA	ACGTGAGGTTTCCAAATGCT	50.0	45.0	58.0	58.0	2213-2625 bp	1.0	91.5
accD-ycf1_p4	AGTATGGGATCGGTAGTGGG	AAGACATCTGAATCCACGGC	55.0	50.0	58.0	58.0	1968 bp	0.25	49.7
accD-ycf1_p5	GTTCATGGGAGGCAGTATGG	AAGACATCTGAATCCACGGC	55.0	50.0	58.0	58.0	1981 bp	0.25	49.5