Hotspot

atpB-atpE

Genome · 109666-109679 · 14 bp

Back to Chamaecyparis

Pi 0.0357

MarkerSeek score 59.20

Primer available yes

Label atpB-atpE

Feature Metadata

label_name: atpB-atpE
feature_type: igs
region: Genome
length_bp: 14
variable_sites: 1
pi: 0.0357
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-atpE_p1	TGGTCTACCCGAACAGTCTT	GGTGGAAATGCGCCTAGTTA	50.0	50.0	58.0	58.0	652-723 bp	1.0	86.6
atpB-atpE_p2	AGCACGGAAATGGGTTCTTT	GGTGGAAATGCGCCTAGTTA	45.0	50.0	58.0	58.0	1146-1217 bp	1.0	85.8
atpB-atpE_p3	TGGTCTACCCGAACAGTCTT	AAACCACCCATCAAAGCCAT	50.0	45.0	58.0	58.0	284-293 bp	1.0	84.2
atpB-atpE_p4	AGCACGGAAATGGGTTCTTT	AAACCACCCATCAAAGCCAT	45.0	45.0	58.0	58.0	778-787 bp	1.0	82.0
atpB-atpE_p5	AGCACGGAAATGGGTTCTTT	TGCTTGTCTCTTGCCTTCAG	45.0	50.0	58.0	58.1	917-926 bp	1.0	81.1