Hotspot

ccsA-ndhJ

Genome · 104348-104682 · 335 bp

Back to Chamaecyparis

Pi 0.0404

MarkerSeek score 67.10

Primer available yes

Label ccsA-ndhJ

Feature Metadata

label_name: ccsA-ndhJ
feature_type: igs
region: Genome
length_bp: 335
variable_sites: 27
pi: 0.0404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhJ_p1	AGAGAAAAAGAGACATGAGCTGA	AGAAAGTTGGATTGGGTGGC	39.1	50.0	57.5	58.4	461-658 bp	1.0	53.6
ccsA-ndhJ_p2	AGAGAAAAAGAGACATGAGCTGA	GCCAGAAAGTTGGATTGGGT	39.1	50.0	57.5	58.4	464-661 bp	1.0	53.5
ccsA-ndhJ_p3	AGAGAAAAAGAGACATGAGCTGA	TGCCAGAAAGTTGGATTGGG	39.1	50.0	57.5	58.4	465-662 bp	1.0	53.5
ccsA-ndhJ_p4	GAGAAAAAGAGACATGAGCTGA	AGAAAGTTGGATTGGGTGGC	40.9	50.0	56.1	58.4	460-657 bp	1.0	50.3
ccsA-ndhJ_p5	GAGAAAAAGAGACATGAGCTGA	GCCAGAAAGTTGGATTGGGT	40.9	50.0	56.1	58.4	463-660 bp	1.0	50.3