Hotspot

petA-psbJ

Genome · 95853-96700 · 848 bp

Back to Chamaecyparis

Pi 0.0388

MarkerSeek score 83.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 848
variable_sites: 62
pi: 0.0388
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTCGTTTTAGCACAG	CGGAAGGATCCCTCTTTGGT	47.6	55.0	57.9	59.1	1041-1132 bp	1.0	68.4
petA-psbJ_p2	GCATCTGTCGTTTTAGCACAG	GGAAGGATCCCTCTTTGGTT	47.6	50.0	57.9	56.8	1040-1131 bp	1.0	67.4
petA-psbJ_p3	AGCATCTGTCGTTTTAGCACA	CGGAAGGATCCCTCTTTGGT	42.9	55.0	58.2	59.1	1042-1133 bp	1.0	67.4
petA-psbJ_p4	GCATCTGTCGTTTTAGCACA	CGGAAGGATCCCTCTTTGGT	45.0	55.0	56.7	59.1	1041-1132 bp	1.0	67.2
petA-psbJ_p5	GCATCTGTCGTTTTAGCACAG	GGAAGGATCCCTCTTTGGTTG	47.6	52.4	57.9	58.3	1040-1131 bp	1.0	66.9