Hotspot

ndhB-ndhB

Genome · 68274-68909 · 636 bp

Back to Chamaecyparis

Pi 0.0113
MarkerSeek score 57.80
Primer available yes
Label ndhB-ndhB

Feature Metadata

label_name
ndhB-ndhB
feature_type
igs
region
Genome
length_bp
636
variable_sites
13
pi
0.0113
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-ndhB_p1 TTTTCATCAATGGACCCCCG AGTGGCTAAAGCTGAAGCAG 50.0 50.0 58.2 58.2 736-765 bp 1.0 79.2
ndhB-ndhB_p2 ATCAATGGACCCCCGATGTA AGTGGCTAAAGCTGAAGCAG 50.0 50.0 58.2 58.2 731-760 bp 1.0 79.0
ndhB-ndhB_p3 TCAATGGACCCCCGATGTAT AGTGGCTAAAGCTGAAGCAG 50.0 50.0 58.2 58.2 730-759 bp 1.0 79.0
ndhB-ndhB_p4 TTTTCATCAATGGACCCCCG AAAAGCAACGACTGGAGTGG 50.0 50.0 58.2 58.7 694-723 bp 1.0 75.3
ndhB-ndhB_p5 TCAATGGACCCCCGATGTAT AAAAGCAACGACTGGAGTGG 50.0 50.0 58.2 58.7 688-717 bp 1.0 75.0