Hotspot

trnV-GAC-rps12

Genome · 65151-66005 · 855 bp

Back to Chamaecyparis

Pi 0.0252

MarkerSeek score 60.10

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: Genome
length_bp: 855
variable_sites: 41
pi: 0.0252
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GACTTTCACCACGTCAAGGT	GTACTCTAGCGACTTTGCGT	50.0	50.0	58.1	57.7	930-954 bp	1.0	85.0
trnV-GAC-rps12_p2	TGACTTTCACCACGTCAAGG	GTACTCTAGCGACTTTGCGT	50.0	50.0	58.1	57.7	931-955 bp	1.0	85.0
trnV-GAC-rps12_p3	GACTTTCACCACGTCAAGGT	TCTAGCGACTTTGCGTAAGG	50.0	50.0	58.1	57.7	926-950 bp	1.0	84.9
trnV-GAC-rps12_p4	TGACTTTCACCACGTCAAGG	TCTAGCGACTTTGCGTAAGG	50.0	50.0	58.1	57.7	927-951 bp	1.0	84.9
trnV-GAC-rps12_p5	GACTTTCACCACGTCAAGGT	TCGTACTCTAGCGACTTTGC	50.0	50.0	58.1	57.5	932-956 bp	1.0	82.8