Hotspot

CDD10_pgr001-trnV-GAC

Genome · 64306-65078 · 773 bp

Back to Chamaecyparis

Pi 0.0808

MarkerSeek score 67.20

Primer available yes

Label CDD10_pgr001-trnV-GAC

Feature Metadata

label_name: CDD10_pgr001-trnV-GAC
feature_type: igs
region: Genome
length_bp: 773
variable_sites: 91
pi: 0.0808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
CDD10_pgr001-trnV-GAC_p1	CCTACAGACACTTTACGCCC	ACCTTGACGTGGTGAAAGTC	55.0	50.0	58.0	58.1	1171-1375 bp	1.0	89.8
CDD10_pgr001-trnV-GAC_p2	CCTACAGACACTTTACGCCC	CCTTGACGTGGTGAAAGTCA	55.0	50.0	58.0	58.1	1170-1374 bp	1.0	89.8
CDD10_pgr001-trnV-GAC_p3	GTGTTCCTCCCGATCTCAAC	ACCTTGACGTGGTGAAAGTC	55.0	50.0	58.0	58.1	1305-1509 bp	1.0	89.2
CDD10_pgr001-trnV-GAC_p4	GCTCCTCAGCCTACCTCATA	ACCTTGACGTGGTGAAAGTC	55.0	50.0	58.0	58.1	1017 bp	0.25	49.6
CDD10_pgr001-trnV-GAC_p5	GCTCCTCAGCCTACCTCATA	CCTTGACGTGGTGAAAGTCA	55.0	50.0	58.0	58.1	1016 bp	0.25	49.6