Hotspot

trnL-UAA-trnF-GAA

Genome · 43712-43984 · 273 bp

Back to Chamaecyparis

Pi 0.0349

MarkerSeek score 72.80

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: Genome
length_bp: 273
variable_sites: 18
pi: 0.0349
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TAGATCGTGAGGGTTCGAGT	TACCAACTGAGCTATCCCGG	50.0	55.0	57.6	58.6	327-350 bp	1.0	79.6
trnL-UAA-trnF-GAA_p2	TAGATCGTGAGGGTTCGAGT	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.6	57.4	337-360 bp	1.0	79.2
trnL-UAA-trnF-GAA_p3	TAGATCGTGAGGGTTCGAGT	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.6	57.4	341-364 bp	1.0	79.1
trnL-UAA-trnF-GAA_p4	TAGATCGTGAGGGTTCGAGT	TTCAGTCCTCTGCTCTACCA	50.0	50.0	57.6	57.4	342-365 bp	1.0	79.1
trnL-UAA-trnF-GAA_p5	TAGATCGTGAGGGTTCGAGT	AGTCCTCTGCTCTACCAACT	50.0	50.0	57.6	57.4	339-362 bp	1.0	79.1