Hotspot

trnE-UUC-psbD

Genome · 28322-29611 · 1290 bp

Back to Chamaecyparis

Pi 0.0332

MarkerSeek score 60.80

Primer available yes

Label trnE-UUC-psbD

Feature Metadata

label_name: trnE-UUC-psbD
feature_type: igs
region: Genome
length_bp: 1290
variable_sites: 79
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbD_p1	CGTTGCCTCCTTGAAAGAGA	GACTTTCCAAGGGCTATAGTCA	50.0	45.5	57.8	57.2	1362-1378 bp	1.0	65.2
trnE-UUC-psbD_p2	GAAAGAGAGATGTCCTGGGC	GACTTTCCAAGGGCTATAGTCA	55.0	45.5	57.7	57.2	1350-1366 bp	1.0	64.0
trnE-UUC-psbD_p3	CGTTGCCTCCTTGAAAGAGA	ACTTTCCAAGGGCTATAGTCA	50.0	42.9	57.8	56.1	1361-1377 bp	1.0	63.6
trnE-UUC-psbD_p4	CGTTGCCTCCTTGAAAGAGA	AGACTTTCCAAGGGCTATAGTC	50.0	45.5	57.8	57.0	1363-1379 bp	1.0	63.0
trnE-UUC-psbD_p5	GAAAGAGAGATGTCCTGGGC	ACTTTCCAAGGGCTATAGTCA	55.0	42.9	57.7	56.1	1349-1365 bp	1.0	62.5