Hotspot

trnR-UCU-atpA

Genome · 10009-10048 · 40 bp

Back to Chamaecyparis

Pi 0.0375

MarkerSeek score 58.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: Genome
length_bp: 40
variable_sites: 3
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAGCGTCCATCGTCTAATGG	CTGGGACATTCACGGAACAA	50.0	50.0	57.8	58.1	203 bp	1.0	78.5
trnR-UCU-atpA_p2	AAGCGTCCATCGTCTAATGG	TGGGACATTCACGGAACAAG	50.0	50.0	57.8	58.1	202 bp	1.0	78.5
trnR-UCU-atpA_p3	AAGCGTCCATCGTCTAATGG	TCTACTGGGACATTCACGGA	50.0	50.0	57.8	57.8	207 bp	1.0	77.6
trnR-UCU-atpA_p4	AAGCGTCCATCGTCTAATGG	ACATTCACGGAACAAGCAGA	50.0	45.0	57.8	57.7	198 bp	1.0	77.4
trnR-UCU-atpA_p5	AAGCGTCCATCGTCTAATGG	GACATTCACGGAACAAGCAG	50.0	50.0	57.8	57.4	199 bp	1.0	74.6