Hotspot

psbK-psbI

Genome · 7368-7922 · 555 bp

Back to Chamaecyparis

Pi 0.0127

MarkerSeek score 58.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 555
variable_sites: 14
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCTGCCGTAAGTTTTCG	TCTTTACGCCCAGGATTTCG	50.0	50.0	58.0	58.0	686-687 bp	1.0	81.7
psbK-psbI_p2	ATTGCCTGAAGCTTATGCGA	TCTTTACGCCCAGGATTTCG	45.0	50.0	58.0	58.0	774-775 bp	1.0	81.5
psbK-psbI_p3	TGCCTGAAGCTTATGCGATT	TCTTTACGCCCAGGATTTCG	45.0	50.0	58.0	58.0	772-773 bp	1.0	81.5
psbK-psbI_p4	TTGCCTGAAGCTTATGCGAT	TCTTTACGCCCAGGATTTCG	45.0	50.0	58.0	58.0	773-774 bp	1.0	81.5
psbK-psbI_p5	AGCTGCCGTAAGTTTTCGAT	TCTTTACGCCCAGGATTTCG	45.0	50.0	57.9	58.0	684-685 bp	1.0	81.3