primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	psbK-psbI	1	CAAGCTGCCGTAAGTTTTCG	TCTTTACGCCCAGGATTTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068070	7368	7922	686-687	1.000000	81.7
psbK-psbI_p2	psbK-psbI	2	ATTGCCTGAAGCTTATGCGA	TCTTTACGCCCAGGATTTCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061228	7368	7922	774-775	1.000000	81.5
psbK-psbI_p3	psbK-psbI	3	TGCCTGAAGCTTATGCGATT	TCTTTACGCCCAGGATTTCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061228	7368	7922	772-773	1.000000	81.5
psbK-psbI_p4	psbK-psbI	4	TTGCCTGAAGCTTATGCGAT	TCTTTACGCCCAGGATTTCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.061228	7368	7922	773-774	1.000000	81.5
psbK-psbI_p5	psbK-psbI	5	AGCTGCCGTAAGTTTTCGAT	TCTTTACGCCCAGGATTTCG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.113304	7368	7922	684-685	1.000000	81.3
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AAGCGTCCATCGTCTAATGG	CTGGGACATTCACGGAACAA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.334192	10009	10048	203-203	1.000000	78.5
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AAGCGTCCATCGTCTAATGG	TGGGACATTCACGGAACAAG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.334192	10009	10048	202-202	1.000000	78.5
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AAGCGTCCATCGTCTAATGG	TCTACTGGGACATTCACGGA	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.450450	10009	10048	207-207	1.000000	77.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AAGCGTCCATCGTCTAATGG	ACATTCACGGAACAAGCAGA	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.479614	10009	10048	198-198	1.000000	77.4
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AAGCGTCCATCGTCTAATGG	GACATTCACGGAACAAGCAG	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.826549	10009	10048	199-199	1.000000	74.6
trnE-UUC-psbD_p1	trnE-UUC-psbD	1	CGTTGCCTCCTTGAAAGAGA	GACTTTCCAAGGGCTATAGTCA	20	22	50.0	45.5	57.8	57.2	0.0	6.7	2.925099	28322	29611	1362-1378	1.000000	65.2
trnE-UUC-psbD_p2	trnE-UUC-psbD	2	GAAAGAGAGATGTCCTGGGC	GACTTTCCAAGGGCTATAGTCA	20	22	55.0	45.5	57.7	57.2	0.0	6.7	3.079234	28322	29611	1350-1366	1.000000	64.0
trnE-UUC-psbD_p3	trnE-UUC-psbD	3	CGTTGCCTCCTTGAAAGAGA	ACTTTCCAAGGGCTATAGTCA	20	21	50.0	42.9	57.8	56.1	0.0	6.7	3.119392	28322	29611	1361-1377	1.000000	63.6
trnE-UUC-psbD_p4	trnE-UUC-psbD	4	CGTTGCCTCCTTGAAAGAGA	AGACTTTCCAAGGGCTATAGTC	20	22	50.0	45.5	57.8	57.0	0.0	0.8	3.200854	28322	29611	1363-1379	1.000000	63.0
trnE-UUC-psbD_p5	trnE-UUC-psbD	5	GAAAGAGAGATGTCCTGGGC	ACTTTCCAAGGGCTATAGTCA	20	21	55.0	42.9	57.7	56.1	0.0	6.7	3.273528	28322	29611	1349-1365	1.000000	62.5
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	TAGATCGTGAGGGTTCGAGT	TACCAACTGAGCTATCCCGG	20	20	50.0	55.0	57.6	58.6	0.0	0.0	1.004989	43712	43984	327-350	1.000000	79.6
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	TAGATCGTGAGGGTTCGAGT	TCCTCTGCTCTACCAACTGA	20	20	50.0	50.0	57.6	57.4	0.0	0.0	1.019905	43712	43984	337-360	1.000000	79.2
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	TAGATCGTGAGGGTTCGAGT	TCAGTCCTCTGCTCTACCAA	20	20	50.0	50.0	57.6	57.4	0.0	0.0	1.019905	43712	43984	341-364	1.000000	79.1
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	TAGATCGTGAGGGTTCGAGT	TTCAGTCCTCTGCTCTACCA	20	20	50.0	50.0	57.6	57.4	0.0	0.0	1.019905	43712	43984	342-365	1.000000	79.1
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	TAGATCGTGAGGGTTCGAGT	AGTCCTCTGCTCTACCAACT	20	20	50.0	50.0	57.6	57.4	0.0	0.0	1.029128	43712	43984	339-362	1.000000	79.1
CDD10_pgr001-trnV-GAC_p1	CDD10_pgr001-trnV-GAC	1	CCTACAGACACTTTACGCCC	ACCTTGACGTGGTGAAAGTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.056505	64306	65078	1171-1375	1.000000	89.8
CDD10_pgr001-trnV-GAC_p2	CDD10_pgr001-trnV-GAC	2	CCTACAGACACTTTACGCCC	CCTTGACGTGGTGAAAGTCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.057866	64306	65078	1170-1374	1.000000	89.8
CDD10_pgr001-trnV-GAC_p3	CDD10_pgr001-trnV-GAC	3	GTGTTCCTCCCGATCTCAAC	ACCTTGACGTGGTGAAAGTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.062075	64306	65078	1305-1509	1.000000	89.2
CDD10_pgr001-trnV-GAC_p4	CDD10_pgr001-trnV-GAC	4	GCTCCTCAGCCTACCTCATA	ACCTTGACGTGGTGAAAGTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.053040	64306	65078	1017-1017	0.250000	49.6
CDD10_pgr001-trnV-GAC_p5	CDD10_pgr001-trnV-GAC	5	GCTCCTCAGCCTACCTCATA	CCTTGACGTGGTGAAAGTCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.054401	64306	65078	1016-1016	0.250000	49.6
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GACTTTCACCACGTCAAGGT	GTACTCTAGCGACTTTGCGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.310039	65151	66005	930-954	1.000000	85.0
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	TGACTTTCACCACGTCAAGG	GTACTCTAGCGACTTTGCGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.311400	65151	66005	931-955	1.000000	85.0
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	GACTTTCACCACGTCAAGGT	TCTAGCGACTTTGCGTAAGG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.323904	65151	66005	926-950	1.000000	84.9
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	TGACTTTCACCACGTCAAGG	TCTAGCGACTTTGCGTAAGG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.325265	65151	66005	927-951	1.000000	84.9
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	GACTTTCACCACGTCAAGGT	TCGTACTCTAGCGACTTTGC	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.589881	65151	66005	932-956	1.000000	82.8
ndhB-ndhB_p1	ndhB-ndhB	1	TTTTCATCAATGGACCCCCG	AGTGGCTAAAGCTGAAGCAG	20	20	50.0	50.0	58.2	58.2	0.0	19.1	0.335351	68274	68909	736-765	1.000000	79.2
ndhB-ndhB_p2	ndhB-ndhB	2	ATCAATGGACCCCCGATGTA	AGTGGCTAAAGCTGAAGCAG	20	20	50.0	50.0	58.2	58.2	0.0	19.1	0.366929	68274	68909	731-760	1.000000	79.0
ndhB-ndhB_p3	ndhB-ndhB	3	TCAATGGACCCCCGATGTAT	AGTGGCTAAAGCTGAAGCAG	20	20	50.0	50.0	58.2	58.2	0.0	19.1	0.366929	68274	68909	730-759	1.000000	79.0
ndhB-ndhB_p4	ndhB-ndhB	4	TTTTCATCAATGGACCCCCG	AAAAGCAACGACTGGAGTGG	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.844995	68274	68909	694-723	1.000000	75.3
ndhB-ndhB_p5	ndhB-ndhB	5	TCAATGGACCCCCGATGTAT	AAAAGCAACGACTGGAGTGG	20	20	50.0	50.0	58.2	58.7	0.0	0.0	0.876573	68274	68909	688-717	1.000000	75.0
petA-psbJ_p1	petA-psbJ	1	GCATCTGTCGTTTTAGCACAG	CGGAAGGATCCCTCTTTGGT	21	20	47.6	55.0	57.9	59.1	0.0	8.6	2.196743	95853	96700	1041-1132	1.000000	68.4
petA-psbJ_p2	petA-psbJ	2	GCATCTGTCGTTTTAGCACAG	GGAAGGATCCCTCTTTGGTT	21	20	47.6	50.0	57.9	56.8	0.0	8.6	2.314364	95853	96700	1040-1131	1.000000	67.4
petA-psbJ_p3	petA-psbJ	3	AGCATCTGTCGTTTTAGCACA	CGGAAGGATCCCTCTTTGGT	21	20	42.9	55.0	58.2	59.1	0.0	8.6	2.321201	95853	96700	1042-1133	1.000000	67.4
petA-psbJ_p4	petA-psbJ	4	GCATCTGTCGTTTTAGCACA	CGGAAGGATCCCTCTTTGGT	20	20	45.0	55.0	56.7	59.1	0.0	8.6	2.338272	95853	96700	1041-1132	1.000000	67.2
petA-psbJ_p5	petA-psbJ	5	GCATCTGTCGTTTTAGCACAG	GGAAGGATCCCTCTTTGGTTG	21	21	47.6	52.4	57.9	58.3	0.0	8.6	2.381223	95853	96700	1040-1131	1.000000	66.9
rps12-clpP_p1	rps12-clpP	1	CGCACATACTCCTTTACGCT	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.390965	101458	102566	1178-1291	1.000000	87.6
rps12-clpP_p2	rps12-clpP	2	TCGCACATACTCCTTTACGC	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.396263	101458	102566	1179-1292	1.000000	87.5
rps12-clpP_p3	rps12-clpP	3	GCTGTCTCGCGGTTCTAATA	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	57.5	58.3	6.1	0.0	0.800487	101458	102566	1113-1226	1.000000	84.7
rps12-clpP_p4	rps12-clpP	4	CACATACACCCTCGCACATA	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	57.4	58.3	0.0	0.0	0.908659	101458	102566	1190-1303	1.000000	83.4
rps12-clpP_p5	rps12-clpP	5	ACATACACCCTCGCACATAC	GCAGGTTGTTTTTCTAGCGC	20	20	50.0	50.0	57.4	58.3	0.0	0.0	0.910439	101458	102566	1189-1302	1.000000	83.4
ccsA-ndhJ_p1	ccsA-ndhJ	1	AGAGAAAAAGAGACATGAGCTGA	AGAAAGTTGGATTGGGTGGC	23	20	39.1	50.0	57.5	58.4	0.0	0.0	3.848054	104348	104682	461-658	1.000000	53.6
ccsA-ndhJ_p2	ccsA-ndhJ	2	AGAGAAAAAGAGACATGAGCTGA	GCCAGAAAGTTGGATTGGGT	23	20	39.1	50.0	57.5	58.4	0.0	0.0	3.848054	104348	104682	464-661	1.000000	53.5
ccsA-ndhJ_p3	ccsA-ndhJ	3	AGAGAAAAAGAGACATGAGCTGA	TGCCAGAAAGTTGGATTGGG	23	20	39.1	50.0	57.5	58.4	0.0	0.0	3.849237	104348	104682	465-662	1.000000	53.5
ccsA-ndhJ_p4	ccsA-ndhJ	4	GAGAAAAAGAGACATGAGCTGA	AGAAAGTTGGATTGGGTGGC	22	20	40.9	50.0	56.1	58.4	0.0	0.0	4.251687	104348	104682	460-657	1.000000	50.3
ccsA-ndhJ_p5	ccsA-ndhJ	5	GAGAAAAAGAGACATGAGCTGA	GCCAGAAAGTTGGATTGGGT	22	20	40.9	50.0	56.1	58.4	0.0	0.0	4.251687	104348	104682	463-660	1.000000	50.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGAGAATGCCAGAATCGGAA	GAGAAGGTCTACGGTTCGAG	20	20	45.0	55.0	56.9	57.2	0.0	0.0	1.907174	106395	107069	775-787	1.000000	70.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTGAGAATGCCAGAATCGGA	GAGAAGGTCTACGGTTCGAG	20	20	45.0	55.0	56.9	57.2	0.0	0.0	1.907174	106395	107069	776-788	1.000000	70.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGAGAATGCCAGAATCGGAA	CGAGAAGGTCTACGGTTCGA	20	20	45.0	55.0	56.9	58.9	0.0	0.0	1.996408	106395	107069	776-788	1.000000	70.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTGAGAATGCCAGAATCGGA	CGAGAAGGTCTACGGTTCGA	20	20	45.0	55.0	56.9	58.9	0.0	0.0	1.996408	106395	107069	777-789	1.000000	70.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TTGAGAATGCCAGAATCGGAA	GAGAAGGTCTACGGTTCGAG	21	20	42.9	55.0	57.7	57.2	0.0	0.0	2.177521	106395	107069	776-788	1.000000	68.7
atpB-atpE_p1	atpB-atpE	1	TGGTCTACCCGAACAGTCTT	GGTGGAAATGCGCCTAGTTA	20	20	50.0	50.0	58.0	58.0	0.0	4.2	0.038051	109666	109679	652-723	1.000000	86.6
atpB-atpE_p2	atpB-atpE	2	AGCACGGAAATGGGTTCTTT	GGTGGAAATGCGCCTAGTTA	20	20	45.0	50.0	58.0	58.0	0.0	4.2	0.037304	109666	109679	1146-1217	1.000000	85.8
atpB-atpE_p3	atpB-atpE	3	TGGTCTACCCGAACAGTCTT	AAACCACCCATCAAAGCCAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.033932	109666	109679	284-293	1.000000	84.2
atpB-atpE_p4	atpB-atpE	4	AGCACGGAAATGGGTTCTTT	AAACCACCCATCAAAGCCAT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.033185	109666	109679	778-787	1.000000	82.0
atpB-atpE_p5	atpB-atpE	5	AGCACGGAAATGGGTTCTTT	TGCTTGTCTCTTGCCTTCAG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.116409	109666	109679	917-926	1.000000	81.1
accD-ycf1_p1	accD-ycf1	1	AGTATGGGATCGGTAGTGGG	ACGTGAGGTTTCCAAATGCT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.029866	114697	116162	2197-2609	1.000000	91.8
accD-ycf1_p2	accD-ycf1	2	GTTCATGGGAGGCAGTATGG	ACGTGAGGTTTCCAAATGCT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.045871	114697	116162	2210-2622	1.000000	91.6
accD-ycf1_p3	accD-ycf1	3	TCAGTTCATGGGAGGCAGTA	ACGTGAGGTTTCCAAATGCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.067534	114697	116162	2213-2625	1.000000	91.5
accD-ycf1_p4	accD-ycf1	4	AGTATGGGATCGGTAGTGGG	AAGACATCTGAATCCACGGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.040961	114697	116162	1968-1968	0.250000	49.7
accD-ycf1_p5	accD-ycf1	5	GTTCATGGGAGGCAGTATGG	AAGACATCTGAATCCACGGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.056966	114697	116162	1981-1981	0.250000	49.5
ycf1_p1	ycf1	1	CATCTCTTCCTTCTGACCGC	GGCGGCTTGGTATTGTTCTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.099434	123021	123140	892-964	1.000000	87.2
ycf1_p2	ycf1	2	GTTGCTGGACGAAATGAAGC	GGCGGCTTGGTATTGTTCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063577	123021	123140	962-1034	1.000000	87.0
ycf1_p3	ycf1	3	GTTGCTGGACGAAATGAAGC	GACTTGGGTCGAACCACTTT	20	20	50.0	50.0	58.0	58.0	0.0	25.1	0.067725	123021	123140	1391-1466	1.000000	86.0
ycf1_p4	ycf1	4	CTCCCCGTACAATGAGCTTC	GGCGGCTTGGTATTGTTCTA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.091355	123021	123140	873-873	0.250000	49.3
ycf1_p5	ycf1	5	CTCCCCGTACAATGAGCTTC	GACTTGGGTCGAACCACTTT	20	20	55.0	50.0	58.1	58.0	0.0	25.1	0.095503	123021	123140	1302-1302	0.250000	49.2