Hotspot

ycf1

SSC · 108484-113547 · 5064 bp

Back to Chaetoseris

Pi 0.000658

MarkerSeek score 37.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5064
variable_sites: 6
pi: 0.000658
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCACAGAAACATCTGAACT	TATGACCATCGAGGGGGTTT	40.0	50.0	55.2	58.1	254 bp	1.0	57.4
ycf1_p2	TTGCACAGAAACATCTGAACT	TATGACCATCGAGGGGGTTT	38.1	50.0	56.0	58.1	255 bp	1.0	55.8
ycf1_p3	ATTGCACAGAAACATCTGAACT	TATGACCATCGAGGGGGTTT	36.4	50.0	56.5	58.1	256 bp	1.0	52.0
ycf1_p4	TGCACAGAAACATCTGAACT	GTATGACCATCGAGGGGGTT	40.0	55.0	55.2	58.9	255 bp	1.0	51.3
ycf1_p5	TGCACAGAAACATCTGAACT	TTGTATGACCATCGAGGGGG	40.0	55.0	55.2	58.9	257 bp	1.0	51.3