Hotspot

ndhI

SSC · 117724-118224 · 501 bp

Back to Chaetoseris

Pi 0.0012

MarkerSeek score 38.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 1
pi: 0.0012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TGTTCCCAACTTATTTCACTGT	TAAAACAGGATTCTCGCCCC	36.4	50.0	55.8	57.6	695 bp	1.0	43.5
ndhI_p2	ACTTGTTCCCAACTTATTTCACTG	TAAAACAGGATTCTCGCCCC	37.5	50.0	57.6	57.6	698 bp	1.0	41.7
ndhI_p3	CTTGTTCCCAACTTATTTCACTGT	TAAAACAGGATTCTCGCCCC	37.5	50.0	57.6	57.6	697 bp	1.0	41.7
ndhI_p4	TGTTCCCAACTTATTTCACTGT	AGGATTCTCGCCCCCTAATC	36.4	55.0	55.8	58.6	689 bp	1.0	41.6
ndhI_p5	TTGTTCCCAACTTATTTCACTGT	TAAAACAGGATTCTCGCCCC	34.8	50.0	56.6	57.6	696 bp	1.0	41.3