Hotspot

ndhD

SSC · 120188-121708 · 1521 bp

Back to Chaetoseris

Pi 0.00101

MarkerSeek score 43.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 3
pi: 0.00101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCCAAAACCTGTGCTCAAAA	TCTTTTCATTAGTCAACCAACTCT	45.0	33.3	57.7	56.2	1635 bp	1.0	40.1
ndhD_p2	GAAAACCCTACTTGAGCCCA	TCTTTTCATTAGTCAACCAACTCT	50.0	33.3	57.4	56.2	1669 bp	1.0	40.1
ndhD_p3	GCCAAAACCTGTGCTCAAAA	TCTTTTCATTAGTCAACCAACTCTT	45.0	32.0	57.7	56.9	1635 bp	1.0	40.1
ndhD_p4	GCCAAAACCTGTGCTCAAAA	TTCTTTTCATTAGTCAACCAACTCT	45.0	32.0	57.7	56.9	1636 bp	1.0	40.1
ndhD_p5	GCCAAAACCTGTGCTCAAAA	TCTTTTCATTAGTCAACCAACTC	45.0	34.8	57.7	54.8	1635 bp	1.0	40.1