Hotspot

clpP

LSC · 69706-71746 · 2041 bp

Back to Chaetoseris

Pi 0.000621

MarkerSeek score 42.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2041
variable_sites: 2
pi: 0.000621
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGATGCGAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	45.0	45.0	57.9	56.9	2127-2128 bp	1.0	70.6
clpP_p2	CTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2154-2155 bp	1.0	70.1
clpP_p3	AACTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2156-2157 bp	1.0	70.1
clpP_p4	GAAACTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.9	56.9	2158-2159 bp	1.0	70.1
clpP_p5	TATGAAACTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	50.0	45.0	58.1	56.9	2161-2162 bp	1.0	70.0