Hotspot

ccsA

SSC · 121980-122948 · 969 bp

Back to Chaetoseris

Pi 0.00055

MarkerSeek score 40.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTTGATTCACACGGTTGTCG	GCGGCATGACATCTTCTAAA	50.0	45.0	57.4	56.2	1180 bp	1.0	61.2
ccsA_p2	CTTGATTCACACGGTTGTCG	GCGGCATGACATCTTCTAAAA	50.0	42.9	57.4	57.0	1180 bp	1.0	59.2
ccsA_p3	CTTGATTCACACGGTTGTCG	GCGGCATGACATCTTCTAAAAG	50.0	45.5	57.4	58.1	1180 bp	1.0	59.0
ccsA_p4	TGACTTGATTCACACGGTTGT	GCGGCATGACATCTTCTAAA	42.9	45.0	58.1	56.2	1183 bp	1.0	57.1
ccsA_p5	ACTTGACTTGATTCACACGGT	GCGGCATGACATCTTCTAAA	42.9	45.0	57.8	56.2	1186 bp	1.0	56.3