Hotspot

ycf3-trnS-GCU

LSC · 43996-44906 · 911 bp

Back to Chaetoseris

Pi 0.000366

MarkerSeek score 43.40

Primer available yes

Label ycf3-trnS-GCU

Feature Metadata

label_name: ycf3-trnS-GCU
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 1
pi: 0.000366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GCU_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	1021 bp	1.0	70.5
ycf3-trnS-GCU_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	971 bp	1.0	70.2
ycf3-trnS-GCU_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	1009 bp	1.0	70.0
ycf3-trnS-GCU_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	973 bp	1.0	69.7
ycf3-trnS-GCU_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	999 bp	1.0	68.6