Hotspot

trnT-GGU-psbD

LSC · 31119-32356 · 1238 bp

Back to Chaetoseris

Pi 0

MarkerSeek score 49.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1369-1378 bp	1.0	78.9
trnT-GGU-psbD_p2	TCCATGAACCAAGCCCTTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1425-1434 bp	1.0	77.8
trnT-GGU-psbD_p3	TTCCATGAACCAAGCCCTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1426-1435 bp	1.0	77.8
trnT-GGU-psbD_p4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1359-1368 bp	1.0	77.8
trnT-GGU-psbD_p5	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1367-1376 bp	1.0	77.7