Hotspot

matK-trnK

LSC · 3585-4313 · 729 bp

Back to Chaetoseris

Pi 0.000823

MarkerSeek score 40.50

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: LSC
length_bp: 729
variable_sites: 1
pi: 0.000823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.5	58.2	861 bp	1.0	58.8
matK-trnK_p2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.5	58.2	860 bp	1.0	58.8
matK-trnK_p3	AGAAGTGGTGTTGTTGAGATCT	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.3	58.2	808 bp	1.0	57.2
matK-trnK_p4	AGAAGTGGTGTTGTTGAGATCT	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.3	58.2	807 bp	1.0	57.2
matK-trnK_p5	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	40.9	50.0	57.5	58.4	876 bp	1.0	57.0