Hotspot

rpoC2-petL

Genome · 102817-103621 · 805 bp

Back to Chaetophoropsis

Pi 0.242

MarkerSeek score 79.70

Primer available yes

Label rpoC2-petL

Feature Metadata

label_name: rpoC2-petL
feature_type: igs
region: Genome
length_bp: 805
variable_sites: 193
pi: 0.242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-petL_p1	AGAAATGTTTCCTCGAGCCC	GTTGTGTGGGGGCAGAATAA	50.0	50.0	57.9	58.1	1784 bp	0.5	58.4
rpoC2-petL_p2	AGAAATGTTTCCTCGAGCCC	TGTGTGGGGGCAGAATAAAC	50.0	50.0	57.9	58.1	1782 bp	0.5	58.4
rpoC2-petL_p3	AACCTCGCATCGAAACAAGT	TGTGTGGGGGCAGAATAAAC	45.0	50.0	58.1	58.1	1813 bp	0.5	58.4
rpoC2-petL_p4	ACCTCGCATCGAAACAAGTT	GTTGTGTGGGGGCAGAATAA	45.0	50.0	58.1	58.1	1814 bp	0.5	58.4
rpoC2-petL_p5	ACCTCGCATCGAAACAAGTT	TGTGTGGGGGCAGAATAAAC	45.0	50.0	58.1	58.1	1812 bp	0.5	58.4