Hotspot

rpoBb-atpE

Genome · 93255-93948 · 694 bp

Back to Chaetophoropsis

Pi 0.333

MarkerSeek score 82.40

Primer available yes

Label rpoBb-atpE

Feature Metadata

label_name: rpoBb-atpE
feature_type: igs
region: Genome
length_bp: 694
variable_sites: 224
pi: 0.333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb-atpE_p1	CCCCCAATACTAGAGGCAGA	TTCCCAGAACCAGCCATCTA	55.0	50.0	57.9	58.0	1431 bp	0.5	58.9
rpoBb-atpE_p2	CCCCCAATACTAGAGGCAGA	AGCTTGTTCTGGGAAAACGT	55.0	45.0	57.9	57.9	1605 bp	0.5	58.8
rpoBb-atpE_p3	CCCCCAATACTAGAGGCAGA	TTTTCCCAGAACCAGCCATC	55.0	50.0	57.9	58.1	1433 bp	0.5	58.6
rpoBb-atpE_p4	GCTTGAACCCAGTCTCCTTC	TTCCCAGAACCAGCCATCTA	55.0	50.0	58.2	58.0	1392 bp	0.5	58.2
rpoBb-atpE_p5	GCTTGAACCCAGTCTCCTTC	AGCTTGTTCTGGGAAAACGT	55.0	45.0	58.2	57.9	1566 bp	0.5	58.1