Hotspot

ycf4-rpoA

Genome · 84256-85426 · 1171 bp

Back to Chaetophoropsis

Pi 0.42

MarkerSeek score 79.40

Primer available yes

Label ycf4-rpoA

Feature Metadata

label_name: ycf4-rpoA
feature_type: igs
region: Genome
length_bp: 1171
variable_sites: 370
pi: 0.42
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-rpoA_p1	TTTGCAAGTTCAGCAGCTTG	AAGTTGCTTGGTCATGGGAG	45.0	50.0	57.8	58.1	2088 bp	0.5	57.6
ycf4-rpoA_p2	TTTGCAAGTTCAGCAGCTTG	AGAAGTTGCTTGGTCATGGG	45.0	50.0	57.8	58.1	2090 bp	0.5	57.6
ycf4-rpoA_p3	GCAAGTTCAGCAGCTTGTTT	AAGTTGCTTGGTCATGGGAG	45.0	50.0	57.8	58.1	2085 bp	0.5	57.6
ycf4-rpoA_p4	GCAAGTTCAGCAGCTTGTTT	AGAAGTTGCTTGGTCATGGG	45.0	50.0	57.8	58.1	2087 bp	0.5	57.6
ycf4-rpoA_p5	AGGAAAACCCCAGCGAAAAA	AAGTTGCTTGGTCATGGGAG	45.0	50.0	58.2	58.1	1894 bp	0.5	57.6