Hotspot

rps12-atpH

Genome · 71792-73988 · 2197 bp

Back to Chaetophoropsis

Pi 0.436

MarkerSeek score 81.70

Primer available yes

Label rps12-atpH

Feature Metadata

label_name: rps12-atpH
feature_type: igs
region: Genome
length_bp: 2197
variable_sites: 895
pi: 0.436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-atpH_p1	ACGTACTCGAGCGACTTTTC	ATTGGTCCTGGTATGGGTCA	50.0	50.0	58.0	58.0	2554 bp	0.5	59.5
rps12-atpH_p2	ACGTACTCGAGCGACTTTTC	GAGGAAAGATGGCAGAGTGG	50.0	55.0	58.0	58.0	2803 bp	0.5	59.4
rps12-atpH_p3	ACGTACTCGAGCGACTTTTC	TTATGCGGTTGAAGGGATCG	50.0	50.0	58.0	58.1	2519 bp	0.5	59.3
rps12-atpH_p4	ACGTACTCGAGCGACTTTTC	GGTTATGCGGTTGAAGGGAT	50.0	50.0	58.0	57.9	2521 bp	0.5	59.3
rps12-atpH_p5	ACTCGACCTCCACGAACTAA	ATTGGTCCTGGTATGGGTCA	50.0	50.0	58.1	58.0	2646 bp	0.5	59.1