Hotspot

rpl16-atpF

Genome · 60028-61813 · 1786 bp

Back to Chaetophoropsis

Pi 0.412

MarkerSeek score 86.10

Primer available yes

Label rpl16-atpF

Feature Metadata

label_name: rpl16-atpF
feature_type: igs
region: Genome
length_bp: 1786
variable_sites: 643
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-atpF_p1	CCTTTCATACGACCACGATGA	TCTTTATTGCTCGTTTTACAGA	47.6	31.8	57.8	53.8	1877-2081 bp	1.0	52.0
rpl16-atpF_p2	TCCTTTCATACGACCACGATG	TCTTTATTGCTCGTTTTACAGA	47.6	31.8	57.8	53.8	1878-2082 bp	1.0	52.0
rpl16-atpF_p3	CCTTTCATACGACCACGATGA	TCTTTATTGCTCGTTTTACAGAA	47.6	30.4	57.8	54.6	1877-2081 bp	1.0	52.0
rpl16-atpF_p4	CCTTTCATACGACCACGATGA	TTCTTTATTGCTCGTTTTACAGA	47.6	30.4	57.8	54.6	1878-2082 bp	1.0	52.0
rpl16-atpF_p5	TCCTTTCATACGACCACGATG	TCTTTATTGCTCGTTTTACAGAA	47.6	30.4	57.8	54.6	1878-2082 bp	1.0	52.0