Hotspot

rpl14-rpl16

Genome · 59142-59619 · 478 bp

Back to Chaetophoropsis

Pi 0.286

MarkerSeek score 78.60

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: Genome
length_bp: 478
variable_sites: 111
pi: 0.286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCTACGAACACCTTTACGCG	TGTATATGGAGGGTTTGCGC	50.0	50.0	58.0	58.0	1019 bp	0.5	59.4
rpl14-rpl16_p2	TCTACGAACACCTTTACGCG	CGTATGGGTTCTGGGAAAGG	50.0	55.0	58.0	58.0	856 bp	0.5	59.4
rpl14-rpl16_p3	TCTACGAACACCTTTACGCG	GTTTGCGCTTCAAGCTACAG	50.0	50.0	58.0	58.0	1007 bp	0.5	59.4
rpl14-rpl16_p4	GCCAGTATTATCGGCGACAT	TGTATATGGAGGGTTTGCGC	50.0	50.0	57.9	58.0	851 bp	0.5	59.1
rpl14-rpl16_p5	GCCAGTATTATCGGCGACAT	CGTATGGGTTCTGGGAAAGG	50.0	55.0	57.9	58.0	688 bp	0.5	59.1