Hotspot

ycf35

SSC · 15637-16014 · 378 bp

Back to Chaetoceros

Pi 0.184

MarkerSeek score 78.90

Primer available yes

Label ycf35

Feature Metadata

label_name: ycf35
feature_type: gene
region: SSC
length_bp: 378
variable_sites: 154
pi: 0.184
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35_p1	CCTCCTGGGAAGATAGCTCA	AACCTTCGGAAACAGTTGCT	55.0	45.0	57.9	57.9	1115 bp	0.111111	43.3
ycf35_p2	ACCTCCTGGGAAGATAGCTC	AACCTTCGGAAACAGTTGCT	55.0	45.0	57.9	57.9	1116 bp	0.111111	43.3
ycf35_p3	GACCTCCTGGGAAGATAGCT	AACCTTCGGAAACAGTTGCT	55.0	45.0	57.9	57.9	1117 bp	0.111111	43.3
ycf35_p4	CCTCCTGGGAAGATAGCTCA	TGGAGCCCGTCAACTAGTTA	55.0	50.0	57.9	58.1	1655 bp	0.111111	43.1
ycf35_p5	ACCTCCTGGGAAGATAGCTC	TGGAGCCCGTCAACTAGTTA	55.0	50.0	57.9	58.1	1656 bp	0.111111	43.1