Hotspot

rps16

SSC · 16155-16391 · 237 bp

Back to Chaetoceros

Pi 0.2

MarkerSeek score 72.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: SSC
length_bp: 237
variable_sites: 103
pi: 0.2
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCGTTCCCATTCCAAGCAAA	TGGAGCCCGTCAACTAGTTA	45.0	50.0	58.0	58.1	912 bp	0.111111	43.8
rps16_p2	TTCGTTCCCATTCCAAGCAA	TGGAGCCCGTCAACTAGTTA	45.0	50.0	58.0	58.1	913 bp	0.111111	43.8
rps16_p3	TCGTTCCCATTCCAAGCAAA	AAAAGATCGTCCAGGTCAGC	45.0	50.0	58.0	57.9	1155 bp	0.111111	43.5
rps16_p4	TTCGTTCCCATTCCAAGCAA	AAAAGATCGTCCAGGTCAGC	45.0	50.0	58.0	57.9	1156 bp	0.111111	43.5
rps16_p5	GGGCATGATTGTTCCCAGAA	TGGAGCCCGTCAACTAGTTA	50.0	50.0	58.1	58.1	957 bp	0.111111	42.7