Hotspot

rpoB

LSC · 73740-78002 · 4263 bp

Back to Chaetoceros

Pi 0.162

MarkerSeek score 65.20

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 4263
variable_sites: 1237
pi: 0.162
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TTAGCTGCGCCAGTTGTTTA	AGAATTGGCCATGTTCTCGC	45.0	50.0	58.1	58.9	1163 bp	0.111111	36.3
rpoB_p2	TTAGCTGCGCCAGTTGTTTA	CCATGTTCTCGCGAATTCATG	45.0	47.6	58.1	58.0	1155 bp	0.111111	35.4
rpoB_p3	CTTTTTAGCTGCGCCAGTTG	AGAATTGGCCATGTTCTCGC	50.0	50.0	58.3	58.9	1167 bp	0.111111	34.7
rpoB_p4	TTTCACTTTTTAGCTGCGCC	AGAATTGGCCATGTTCTCGC	45.0	50.0	57.6	58.9	1172 bp	0.111111	33.9
rpoB_p5	CTTTTTAGCTGCGCCAGTTG	CCATGTTCTCGCGAATTCATG	50.0	47.6	58.3	58.0	1159 bp	0.111111	33.8