Hotspot

rpl20

LSC · 8174-8518 · 345 bp

Back to Chaetoceros

Pi 0.147

MarkerSeek score 79.30

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 345
variable_sites: 112
pi: 0.147
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	AAAAGCTGCAGCAAAACGTT	TTAATGTTACAGGAGCGGCC	40.0	50.0	58.0	58.0	1276 bp	0.111111	44.1
rpl20_p2	AAAAAGACAGGCGCAGGTAA	TTAATGTTACAGGAGCGGCC	45.0	50.0	58.0	58.0	1254 bp	0.111111	44.0
rpl20_p3	AAAAGCTGCAGCAAAACGTT	AGTCTACCATGGACTGCTGT	40.0	50.0	58.0	58.1	1213 bp	0.111111	43.9
rpl20_p4	AAAAAGACAGGCGCAGGTAA	AGTCTACCATGGACTGCTGT	45.0	50.0	58.0	58.1	1191 bp	0.111111	43.8
rpl20_p5	CAGTTGATCAAGGGGCTGTT	TTAATGTTACAGGAGCGGCC	50.0	50.0	58.1	58.0	1689 bp	0.111111	43.5