Hotspot

trnP(ugg)-ycf45

LSC · 62147-62629 · 483 bp

Back to Chaetoceros

Pi 0.276

MarkerSeek score 79.80

Primer available yes

Label trnP(ugg)-ycf45

Feature Metadata

label_name: trnP(ugg)-ycf45
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 285
pi: 0.276
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP(ugg)-ycf45_p1	TTAATGTTACAGGAGCGGCC	TTTCGCGTCAAGAGTTTCCA	50.0	45.0	58.0	58.1	1350 bp	0.111111	43.7
trnP(ugg)-ycf45_p2	AGTCTACCATGGACTGCTGT	TTTCGCGTCAAGAGTTTCCA	50.0	45.0	58.1	58.1	1287 bp	0.111111	43.5
trnP(ugg)-ycf45_p3	TTAATGTTACAGGAGCGGCC	CTTGCAGCTCGTACAATTGC	50.0	50.0	58.0	58.1	1795 bp	0.111111	43.3
trnP(ugg)-ycf45_p4	AGTCTACCATGGACTGCTGT	CTTGCAGCTCGTACAATTGC	50.0	50.0	58.1	58.1	1732 bp	0.111111	43.1
trnP(ugg)-ycf45_p5	CCAAGCTGAGCTATATCCCG	TTTCGCGTCAAGAGTTTCCA	55.0	45.0	57.6	58.1	775 bp	0.111111	41.0