Hotspot

rns-trnI(gau)

IRb · 11741-11805 · 65 bp

Back to Chaetoceros

Pi 0.16

MarkerSeek score 80.40

Primer available yes

Label rns-trnI(gau)

Feature Metadata

label_name: rns-trnI(gau)
feature_type: igs
region: IRb
length_bp: 65
variable_sites: 23
pi: 0.16
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(gau)_p1	GACTGGGGTGAAGTCGTAAC	CTGGGCCATTCTGGATTTGA	55.0	50.0	57.9	57.9	199-202 bp	1.0	87.0
rns-trnI(gau)_p2	GGGGTGAAGTCGTAACAAGG	CTGGGCCATTCTGGATTTGA	55.0	50.0	58.2	57.9	195-198 bp	1.0	86.1
rns-trnI(gau)_p3	GTAACCGTACTGGAAGGTGC	CTGGGCCATTCTGGATTTGA	55.0	50.0	58.3	57.9	176-179 bp	1.0	85.5
rns-trnI(gau)_p4	TGACTGGGGTGAAGTCGTAA	CTGGGCCATTCTGGATTTGA	50.0	50.0	58.3	57.9	200-203 bp	1.0	85.3
rns-trnI(gau)_p5	AGGTAACCGTACTGGAAGGT	CTGGGCCATTCTGGATTTGA	50.0	50.0	57.7	57.9	178-181 bp	1.0	85.2