Hotspot

trnS(gcu)-trnD(guc)

LSC · 3046-3105 · 60 bp

Back to Chaetoceros

Pi 0.368

MarkerSeek score 79.70

Primer available yes

Label trnS(gcu)-trnD(guc)

Feature Metadata

label_name: trnS(gcu)-trnD(guc)
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 39
pi: 0.368
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gcu)-trnD(guc)_p1	GGGTTCGAATCCCTGTTTCT	AGACTCGAACTCGCAACTTC	50.0	50.0	57.5	57.9	145-153 bp	1.0	87.4
trnS(gcu)-trnD(guc)_p2	GGGTTCGAATCCCTGTTTCT	GAGACTCGAACTCGCAACTT	50.0	50.0	57.5	57.9	146-154 bp	1.0	87.4
trnS(gcu)-trnD(guc)_p3	GGGTTCGAATCCCTGTTTCT	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.5	58.6	113-121 bp	1.0	83.7
trnS(gcu)-trnD(guc)_p4	GGGTTCGAATCCCTGTTTCT	GACTGACGAGACTCGAACTC	50.0	55.0	57.5	57.5	153-161 bp	1.0	83.5
trnS(gcu)-trnD(guc)_p5	GGGTTCGAATCCCTGTTTCT	GGACTGACGAGACTCGAACT	50.0	55.0	57.5	58.8	154-162 bp	1.0	80.9