Hotspot

ndhF

SSC · 110473-112707 · 2235 bp

Back to Chaerophyllum

Pi 0.0116

MarkerSeek score 49.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 38
pi: 0.0116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAAGCTCATGAAGGCCTACC	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.0	58.3	2359-2397 bp	1.0	80.2
ndhF_p2	GCTCATGAAGGCCTACCATT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.6	58.3	2356-2394 bp	1.0	77.7
ndhF_p3	CGAAGCTCATGAAGGCCTAC	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.4	58.3	2360-2398 bp	1.0	77.2
ndhF_p4	AGCTCATGAAGGCCTACCAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2357-2395 bp	1.0	76.7
ndhF_p5	GAAGCTCATGAAGGCCTACC	GGGTAGGATCCGGTAAATCG	55.0	55.0	58.0	57.3	2357-2395 bp	1.0	76.4