Hotspot

ccsA

SSC · 114775-115749 · 975 bp

Back to Chaerophyllum

Pi 0.0154

MarkerSeek score 53.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 22
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	1475-1485 bp	1.0	82.4
ccsA_p2	TGAAATCGGTAGACACGCTG	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	1474-1484 bp	1.0	82.4
ccsA_p3	GTGAAATCGGTAGACACGCT	GGACCGCGAGAGTTATTTGT	50.0	50.0	58.0	58.0	1474-1484 bp	1.0	82.4
ccsA_p4	TGAAATCGGTAGACACGCTG	GGACCGCGAGAGTTATTTGT	50.0	50.0	58.0	58.0	1473-1483 bp	1.0	82.4
ccsA_p5	GTGAAATCGGTAGACACGCT	AAACGATACGGGACTCAACG	50.0	50.0	58.0	58.0	1885 bp	0.333333	53.2