Hotspot

ndhG-ndhI

SSC · 119144-119462 · 319 bp

Back to Chaerophyllum

Pi 0.0481

MarkerSeek score 72.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 319
variable_sites: 23
pi: 0.0481
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	CGAACAATTTTGAATTTGCCTGA	50.0	34.8	58.0	57.1	415-467 bp	1.0	53.2
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	TCGAACAATTTTGAATTTGCCTG	50.0	34.8	58.0	57.1	416-468 bp	1.0	53.2
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	ACTCGAACAATTTTGAATTTGCC	50.0	34.8	58.0	57.1	418-470 bp	1.0	53.1
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	CTCGAACAATTTTGAATTTGCCTG	50.0	37.5	58.0	58.1	417-469 bp	1.0	51.6
ndhG-ndhI_p5	AAGACCCGATCCCAGAAAGA	CTCGAACAATTTTGAATTTGCCT	50.0	34.8	58.0	56.8	417-469 bp	1.0	51.1