Hotspot

rpl32-trnL-UAG

SSC · 113833-114600 · 768 bp

Back to Chaerophyllum

Pi 0.0334

MarkerSeek score 70.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 768
variable_sites: 36
pi: 0.0334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCCTTAAAAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	807-896 bp	1.0	87.3
rpl32-trnL-UAG_p2	GGGCAGCCTTAAAAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	808-897 bp	1.0	87.3
rpl32-trnL-UAG_p3	GGGCAGCCTTAAAAGCATTG	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.0	58.1	828-917 bp	1.0	86.7
rpl32-trnL-UAG_p4	GGGCAGCCTTAAAAGCATTG	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.0	58.1	830-919 bp	1.0	86.7
rpl32-trnL-UAG_p5	GGGCAGCCTTAAAAGCATTG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	817-906 bp	1.0	86.4